Yazdan Keivany; Sedigheh Mahmoodi; Salar Dorafshan; Mansoureh Malekian
Abstract
So far, 13 species were recently recognized in the Iranian basins. In the present study the variation among A. eichwaldii, A. namaki, A. idignensis, and A. nicolausi, A. qanati A. petrubanarescui has been investigated from morphological and molecular approaches. To study the morphological characteristic, ...
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So far, 13 species were recently recognized in the Iranian basins. In the present study the variation among A. eichwaldii, A. namaki, A. idignensis, and A. nicolausi, A. qanati A. petrubanarescui has been investigated from morphological and molecular approaches. To study the morphological characteristic, 144 specimens of these six species were used, on which 22 morphometrics were measured. Nine meristics characteristics were counted. Analysis of variance (ANOVA), discriminant analysis, and principal component analysis was performed. In the molecular studies, sequences of the mitochondrial gene, cytochrome b from six species were sequenced and analyzed. The morphological results of the data showed a high similarity among species, but could separate A. nicolausi and A. idignensis according to the PC1. Also A. idignensis and A. petrubanarescui are more similar based on the PC2. Moreover, based on meristic characteristics which were relatively similar, again A. nicolausi and A. idignensis could be separated by the first component. The phylogenetic analysis using Maximum Likelihood (ML) and Maximum-Parsimony (MP) methods supported the monophyletic status of the six species, suggesting their status as different species. The results of the molecular analysis showed that A. eichwaldii and A. petrubanarescui have the most similarity (about 99%) and the A. idignensis has the maximum difference (about 85%) with the other species. These results justify the results of morphometric analysis.
Mahmood Banimasani; Yazdan Keivany; Eisa Ebrahimi
Abstract
Abstract Quantifying morphological characteristics of body shape in fishes can help their correct identification as well as understanding of evolutionary history of their different populations. Hence, this study was aimed to compare the morphological characteristics of different population of siahmahi ...
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Abstract Quantifying morphological characteristics of body shape in fishes can help their correct identification as well as understanding of evolutionary history of their different populations. Hence, this study was aimed to compare the morphological characteristics of different population of siahmahi (Capoeta fusca) in inland-water of Iran using geometric morphometric method (GM). In total 122 specimens of Capoeta fusca were collected from three rivers Amirabad, Kalshur, Tabarak. Then, the lateral surface of their left side photographed and seventeen landmark-points defined and digitized using TpsDig2 software to extract body shape data in geometric morphometry. The coordinate data after GPA superimposing, analyzed using principal component analysis (PCA), canonical variate analysis (CVA) and cluster analysis (CA) and body shape pattern of every population was visualized in relation to consensus shape of total populations. Based on the PCA, six factors were above the Joliff line and accounted for more than 79.37% of the variance. The highest variances were related to the head size, body depth, dorsal and anal fin position, dorsal fin base length and caudal peduncle length. The CVA and PCA morphometric of Capoeta fusca indicated that the three populations of Kalshur, Amirabad and Tabarak have meaningful differences and they are separated from each other (p<0.0001). The cluster analysis has also isolated the population of Kalshur from the populations of Amirabad and Tabarak.
Yazdan Keivany; Amirhosein Tahmaseb; Omidvar Farhadian
Abstract
Abstract The objective of this study was to determine the body shape variation among Kura barb species (Barbus spp.) in the Caspian Sea, Urmia and Tigris basins using landmark-based geometric morphometrics. For this purpose, a total of 128 specimens were sampled from the three basins using seine, gill ...
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Abstract The objective of this study was to determine the body shape variation among Kura barb species (Barbus spp.) in the Caspian Sea, Urmia and Tigris basins using landmark-based geometric morphometrics. For this purpose, a total of 128 specimens were sampled from the three basins using seine, gill and cast nets. The left side of each specimen was photographed using digital camera and 14 Landmark points were digitized on two-dimensional images using TpsDig2. Landmark data after a Generalized Procrustes were analyzed using Principal Component Analysis (PCA) and Canonical Variate Analysis (CVA) and the patterns of body shape differences among the studied population were illustrated in deformation grids in relation to consensus configuration of all specimens. The Principal Component Analysis could not differentiate the populations, but CVA displayed some differences among the populations. Habitat-associated morphological divergence, phenotypic plasticity and evolutionary process of body shape change among various populations and also, a relationship between the geographical distance and the degree of morphological divergence among populations in response to environmental factors in their habitat were observed. Thus, each population should be considered as separate genetic and morphological stocks.
Yazdan Keivany; Atta Mouludi Saleh; Seyed Amir Hossein Jalali
Volume 7, Issue 1 , September 2018, , Pages 107-118
Abstract
Abstract In order to evaluate the biometry of chub (Squalius namak, Khaefi et al., 2016) populations in Khaznagh, Ghinercheh, Gharechae, Jajrud and Ghomrud rivers of Lake Namak Basin, 129 specimens were captured by a seine nets in 2010-2011. After anesthetizing in 1% clove oil solution and fixing in ...
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Abstract In order to evaluate the biometry of chub (Squalius namak, Khaefi et al., 2016) populations in Khaznagh, Ghinercheh, Gharechae, Jajrud and Ghomrud rivers of Lake Namak Basin, 129 specimens were captured by a seine nets in 2010-2011. After anesthetizing in 1% clove oil solution and fixing in 10% neutralized formalin, specimens were transferred to the laboratory for further studies. Some 14 meristic characters were counted under a stereomicroscope. To extract data for morphometric characteristics, 19 distances were measured on images in ImageJ software. To reduce the effects of allometric growth, morphometric data were standardized. To analyze the differences among the populations, Kolmogorov-Smirnov, Kruskal-Wallis, ANOVA, Duncan test, PCA, CVA and Cluster analyses were used. The morphometric characteristics separated Gharachai population from Khaznagh and Qomrud in PCA and CVA Analyses. But meristics were overlapping with each other in the studied populations, and there was no significant differences among them. The result showed that some morphometric characteristics are well able to distinguish some populations of S. namak.
E. Haghighy; M. Sattari; S. Dorafshan; Y. Keivany
Volume 3, Issue 4 , July 2015, , Pages 37-46
Abstract
Abstract
Spirlin, Alburnoides eichwaldii, is an abundant riverine fish in the south west Caspian Sea basin. Sixty specimens of Spirlin from Kargan-Rud, and Lamir (30 specimens from each river) were studied and compared from morphological point of view in August 2010.Thirty-one morphometric characters ...
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Abstract
Spirlin, Alburnoides eichwaldii, is an abundant riverine fish in the south west Caspian Sea basin. Sixty specimens of Spirlin from Kargan-Rud, and Lamir (30 specimens from each river) were studied and compared from morphological point of view in August 2010.Thirty-one morphometric characters were measured and thirteen meristictraits were counted on each specimen. Nonparametric methods for comparison of means and Principal Component Analysis (PCA) for separation of populations were used. Among the meristic characters, nine characters were significantly different between the four populations (p<0.05). Among the morphometric and relative morphometric characters, nineteen characters showed significant differences (p<0.05) between the two populations. In the PCA of meristic characters, three factors accounted for 47.5% of variations within populations, including; caudal peduncle scales, scale above lateral line and anal fin ray numbers. Within the morphometric characters, three factors accounted for about 71% of variations within the two populations, including dorsal fin height, pectoral fin base, ventral fin length, caudal peduncle depth, head length, inter orbital width, standard length to pre dorsal length, head length to snout length, head length to eye diameter and head length to inter orbital width. The results showed while morphometric and relative morphometric characters were not useful for separating the two populations and sexes, the meristic characters could relatively separate these two populations.
